Bioinformatic analysis of antibody repertoire development in response to influenza vaccination
10.3760/cma.j.issn.0254-5101.2017.02.009
- VernacularTitle:流感疫苗应答过程中抗体谱发育的生物信息学分析
- Author:
Lu ZHANG
;
Qingchen ZHANG
;
Tianyi QIU
;
Zhiwei CAO
;
Kailin TANG
- Keywords:
Vaccine;
Antibody repertoire;
B cell;
Complementarity-determining region 3 (CDR3);
Immune response
- From:
Chinese Journal of Microbiology and Immunology
2017;37(2):133-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the immunogenomic characteristics of antibody repertoire in re-sponse to influenza vaccine in order to provide a theoretical basis for further development of antibody. Meth-ods Based on a time-series immunoglobulin heavy chain ( IGH) repertoire sequencing dataset, we analyzed the immunogenomic characteristics of antibody repertoire in response to trivalent influenza vaccine ( TIV ) from three aspects which included the features in complementarity-determining region 3 ( CDR3 ) , antibody mutation and VDJ usage. Results The frequency of antibody mutation increased significantly upon vaccina-tion. Analysis of the CDR3 region indicated that polar and aromatic amino acids had a higher preference. The length of CDR3 region in naive B cells followed a normal distribution, while specific CDR3 sequences with 15 to 18 amino acids in length occupied a dominant position after vaccination. In addition, the VDJ us-age altered obviously and IGHV3-7-derived antibody had a significant response to the vaccine. Response in-tensity reached the peak on day 7 and gradually weakened over time. Conclusion Antibody repertoire evolves dynamically to express specific antibody upon vaccination and the characteristics of immune responses at sequence level could be used to evaluate their effectiveness.
