Overexpression of MST1 inhibits the proliferation , migration and invasion of cervical cancer cell line SiHa
- VernacularTitle:MST1过表达抑制宫颈癌细胞系SiHa的增殖、迁移和侵袭
- Author:
Xiaoying ZHENG
;
Yuntao HAO
;
Weijun MA
;
Li HAN
;
Shumin ZHAO
;
Xiangyao LIAN
;
Yujuan ZHANG
- Keywords:
mammalian sterile 20-like kinase 1;
cervical cancer;
proliferation;
migration;
invasion
- From:
Basic & Clinical Medicine
2017;37(3):351-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of MST1 (mammalian sterile 20-like kinase 1,MST1) on the prolifera-tion,migration and invasion of SiHa cervical cancer .Methods Western blot was used to detect the expression of MST1 in cervical epithelial cells H8 and cervical cancer cells SiHa;PJ3H-HA-MST1 was constructed and transfect-ed it to SiHa cells by Lipofectamine TM3000;MST1, Ki-67 and MMP9 protein expression were evaluated by Western blot;While the proliferation ,migration and invasion of SiHa cell were assessed by MTS ,scratch adhesion test and Transwell assay respectively .Results Compared SiHa cells with H 8 cells,MST1 expression in SiHa cells was sig-nificantly lower than that in H8 cells.The plasmid was successfully transfected into SiHa cells , MST1 expression was significantly higher , while the expression of Ki-67 and MMP9 was lower .The proliferation , migration and inva-sion ability were all significantly suppressed .Conclusions Overexpression of MST1 can inhibit the proliferation , migration and invasion of cervical cancer cell line SiHa .
