UVB induces morphological changes and the expression of MMPs in human fibroblasts
- VernacularTitle:紫外线诱导人皮肤成纤维细胞 形态改变及对基质金属蛋白酶表达的影响
- Author:
Yanhua WANG
;
Limin CAI
;
Jing YANG
;
Shuyun ZHENG
- Keywords:
photoaging;
human fibroblast;
telomere;
MMPs
- From:
Basic & Clinical Medicine
2017;37(3):360-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of telomere length and MMPs level in human fibroblasts induced by UVB, and to explore their roles on skin photoaging .Methods Human skin fibroblasts were extracted and cul-tured.The 5th fibroblasts were irradiated by UVB .The morphology of fibroblasts were microscoped , and the length of telomere and the mRNA expression of COL1a1 and hTERT were detected by RT-qPCR.The expression of MMP-3 and MMP-1 were detected by Western blot .Results The fibroblasts gradually became round , wrinkled and disorderly arranged after 30 mJ/cm2 UVB irradiation for 24 h.The mRNA level of COL1a1 and hTERT and the expression of MMP-3 and MMP-1 were significantly increased after UVB irradiation compared with control , and the length of telomere was shortened .Conclusions UVB may frigger the early process of photoaging by the morphologi-cal changes of human skin fibroblasts and increasing the expression of MMP-3 and MMP-1 .
