Nicotine promotes the apoptosis of human umbilical vein endothelial cells
- VernacularTitle:尼古丁促人脐静脉内皮细胞凋亡
- Author:
Xiu LIU
;
Chenyang QIU
;
Chaonan WANG
;
Bao LIU
- Keywords:
human umbilical vein endothelial cells;
nicotine;
apoptosis
- From:
Basic & Clinical Medicine
2017;37(3):391-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To try different concentrations of nicotine promotes the apoptosis of human umbilical vein en-dothelial cells and its mechanism .Methods Human umbilical vein endothelial cells were cultured .According to the concentration of nicotine , grouped as 10 -6 , 10 -7, 10 -8 mol/L, and control.After 24 h of nicotine treat-ment, proliferation/activity were tested by CCK-8 assay;Annexin V-FITC fluorescence staining detects the apoptot-ic cells.Western blot detects the expression of protein Bax , Bcl-2 and PARP-1, to study the mechanism of apopto-sis.Results Compared with control group , in 10 -6 ,10 -7 mol/L groups the proliferation/activity of HUVECs de-creased ( P<0.05 ) .And the number of apoptotic cells increased ( P<0.01 ) .The target proteins expression of 10 -6、10 -7 mol/L groups were changed as follow: Bax increased ( P <0.01 ) , Bcl-2 decreased ( P <0.01 ) and PARP-1 increased ( P<0.01 ) .Conclusions Nicotine may promote the apoptosis of vascular endothelial cells , which accelerates the development of atherosclerotic disease .
