Effects of astragaloside on TRPC6 expression on mouse podocyte induced by TGF-β1

VernacularTitle:黄芪总苷对TGF-β1诱导下足细胞TRPC6表达的影响
Author: Haiting HUANG ; Haohao WU ; Youling QIN ; Xu LIN ; Yanwu YOU ; Pengwei GUO ; Chunrong TANG
Keywords: Podocytes; TGF-β1; TRPC6; AST
From: Chinese Journal of Immunology 2017;33(3):370-373
CountryChina
Language:Chinese
Abstract: Objective:To explore the possible mechanism of astragaloside involved in the mouse podocytes injury induced by TGF-β1 in vitro.Methods:Mouse podocytes were cultured in vitro and then all cell were divided into 5 groups:normal control group , TGF-β1 treatment group ,TGF-β1 treatment +astragaloside low dose group ,TGF-β1 treatment +astragaloside middle dose group and TGF-β1 treatment +astragaloside high dose group.The proliferation rate of each group was investigated by MTT assay ,the expression of TRPC6 protein and mRNA were measured by Western blot and RT-PCR respectively after 48 hours.Results:TGF-β1 can significantly inhibit the proliferation of podocytes ( P<0.05) ,fusions of foot processes or even effaced of podocytes were observed .TGF-β1 could also increase the expression of TRPC6.Astragaloside could reduce the inhibition of TGF-β1 to the proliferain of podocytes significantly ,make the cell shape tend to be normal,and reduce the expression of TRPC6 mRNA and protein with dose-effect relation.Conclusion:TRPC6 play an impor-tant role in the TGF-β1 induecd podocytes injury .Astragaloside can alleviate podocytes injury by reduce the expression of TRPC 6.