Impact of miR-122a on inhibition proliferation of laryngeal carcinoma cell line Hep2
10.3969/j.issn.1000-484X.2017.03.007
- VernacularTitle:miR-122a抑制人喉癌细胞株Hep2细胞增殖的研究
- Author:
Yunhua CHEN
;
Yafeng YU
- Keywords:
Laryngeal carcinoma;
miR-122 a;
Cell cycle;
Hep2 cells
- From:
Chinese Journal of Immunology
2017;33(3):352-355
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of miR-122a on inhibition proliferation of laryngeal carcinoma cell line Hep-2.Meth-ods:The oligomucleotide of miR-122a was transfected into laryngeal carcinoma cell line Hep 2 cells,which were devided into three groups of A(miR-122a transfection),B(miR-122a inhibitor),C(miR-122a-NC inhibitor) and group of D(blank control).The expres-sion of miR-122a was defected by RT-PCR,and relevant protein expression was evaluated by Western blot .The cell proliferation and cell cycle were determined by MTT assy and flow cytometry ,respectively.Results:Compared to group D,miR-122a expression in Hep2 cells was obviously elevated atter miR-122a-transfected.The proliferation of Hep2 cells in group A was significantly inhibited and the cell cycle arrested at G1/G0 phase.The protein expression of CDC42 was downregulated with decreased expressions of CDK 4 and cyclin D1 in group A.Conclusion:miR-122a inhibits the proliferation activity of Hep 2 cells,suggesting that miR-122a can be taken as a po-tential candidate for gene therapy of laryngeal carcinoma .
