Comparative study on two serological detection methods of mycoplasma pneumoniae in children with mycoplasmal pneumonia

Xin Liu; Shasha Guo; Chong Wang; Yuanqi Zhu; Zhenhong Wang; Zhenghai QU

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Comparative study on two serological detection methods of mycoplasma pneumoniae in children with mycoplasmal pneumonia

VernacularTitle:两种血清支原体抗体检测方法在诊断儿童肺炎支原体肺炎中的价值
Author: Xin LIU ; Shasha GUO ; Chong WANG ; Yuanqi ZHU ; Zhenhong WANG ; Zhenghai QU
Keywords: Pneumonia,mycoplasma; Child; Particle agglutination method; Dot immunogold method
From: Chinese Journal of Postgraduates of Medicine 2017;40(3):211-213
CountryChina
Language:Chinese
Abstract: Objective To compare the sensitivity and specificity of dot immunogold method (DIM) and particle agglutination (PA) for the diagnosis of mycoplasma pneumoniae (MP) infection. Methods The 190 serum specimens of 113 children with mycoplasmal pneumonia (infection group) and 50 serum specimens of 50 health children (health group) were tested for MP by PA and DIM- A and B. Results In infection group, the positive rates of DIM- A and B were 82.63% (157/190) and 84.74%(161/190), and there was no statistical difference (χ2 = 0.31, P>0.05); the positive rate of PA (titer ≥1:160) was 70.00%(133/190), the positive rate of PA was significantly lower than that in DIM-A and B, and there were statistical differences (P<0.05). In infection group, with the increase of PA titer, the positive rate of DIM was gradually increased, and there was a correlation between 2 methods (rA=0.972, rB=0.830);the positive rates of DIM- A and B in serum specimens of PA negative were 40.62%(13/32) and 53.12%(17/32), and in the PA titer of 1:5120, there was still a negative result in DIM. In health group, the positive rates of DIM-A and B were 26.00% (13/50) and 28.00% (14/50), and there was no statistical difference (χ2 = 0.66, P>0.05); the positive of PA was 8.00% (4/50), the positive rate of PA was significantly lower than that in DIM- A and B, and there were statistical differences (P<0.05 or<0.01). Conclusions Compared with the PA, DIM has low sensitivity and poor specificity for clinical diagnosis. DIM is not suitable for clinical diagnosis of MP infection.