Promotion of immune response by soluble Tim-3 in vitro and therapeutic potential

VernacularTitle:可溶性Tim-3体外增强免疫应答的作用及机制研究
Author: Ge LI ; Zhiding WANG ; Shuaijie DOU ; Chunmei HOU ; He XIAO ; Renxi WANG ; Guojiang CHEN ; Yan LI ; Gencheng HAN
Keywords: Tim-3; fusion protein; cytokines; U937 cells; Jurkat cells; T-lymphocytes
From:Military Medical Sciences 2017;41(1):33-37
CountryChina
Language:Chinese
Abstract: Objective To evaluate the role of recombinant human soluble Tim-3 (hTim-3-Fc) in regulating immune response.Methods Soluble hTim-3 was incubated with human macrophage cell line U 937, human T cell line Jurkat and normal human PBMC before cytokines secreted by or expressed in different immune cells were analyzed using ELISA , RT-PCR and Western-blotting, respectively.Results Soluble hTim-3 significantly promoted the activation of different immune cells.Our data showed that IL-8 secretion by U937 cells, IL-2 secretion by Jurkat cells , IL-2 and IFN-γsecretion by human PBMCs were all significantly increased .In addition , soluble hTim-3 significantly increased the IFN-α2 and IFN-β1 mRNA expression in U937, Jurkat and PBMCs and increased the phosphorylation of stat-1 in Jurkat and U937 cells.Conclusion Recombinant soluble hTim-3 can significantly promote the activation of immune cells in vitro, which shows its therapeutic potential .