Effects of cyclopamine on endometrial carcinoma (HEC-1 A) and apoptosis
10.7644/j.issn.1674-9960.2017.01.011
- VernacularTitle:环巴胺对子宫内膜癌HEC-1 A细胞存活与凋亡的影响
- Author:
Pingping ZHANG
;
Xue LI
;
Yuanlin LIU
;
Si ZHANG
;
Xiangdong ZHOU
;
Ying TONG
;
Yi ZHANG
- Keywords:
endometrial carcinoma;
cyclopamine;
apoptosis
- From:
Military Medical Sciences
2017;41(1):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cyclopamine (CYP) on endometrial carcinoma (HEC-1A) cell survival and on induction of cell apoptosis .Methods HEC-1A cells were treated with various doses of CYP (0, 5,10, 20 and 40 μmol/L) for 24 h respectively .Then,the inverted microscope was used to observe cell morphology .Cell proliferation and apoptosis were tested by CCK-8 assay and AO/EB bi-labelling assay.The apoptosis rate of HEC-1A was analyzed using flow cytometric analysis , and the key gene expression of Bax and Bcl-2 was detected by quantitative PCR .Results The HEC-1A cells exhibited dramatic morphological changes after treatment with CYP and in a dose-dependent manner .CYP significantly inhibited HEC-1A cell proliferation using CCK8 assays(P<0.05), and induced cell death by AO/EB bi-labelling assay.Moreover,flow cytometry analysis showed that CYP treatment resulted in HEC-1A cell apoptosis, and that a higher concentration of CYP induced severer cell apoptosis (P<0.05).Meanwhile, CYP treated HEC-1A cells exhibited up-regulated expression of Bax and down-regulated expression of Bcl-2 according to Q-PCR.Conclusion Our findings indicatee that CYP can inhibit HEC-1A cell proliferation and induce cell apoptosis .
