Inhibition of gallnut extract on MRSA β-lactamase
10.3969/j.issn.1005-1678.2017.01.009
- VernacularTitle:五倍子粗提物对MRSAβ-内酰胺酶的抑制作用
- Author:
Shanshan PAN
;
Kunpeng XIE
;
Mingjie XIE
- Keywords:
gallnut;
MRSA;
β-lactamase
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(1):39-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study on the inhibitory effect of gallnut extract extract on MRSA β-lactamase. Methods Determination of inhibitory effect of gallnut extract on MRSA3002 by TTC method. β-lactamase was repeated by freezing and thawing method . Synergistic effect of gallnut extract and gentamicin was detected by TTC. Results The MIC and MBC of MRSA3002 by gallnut extract were 8mg/mL and 32mg/mL.Gallnut extract can reduce strains of β-lactamase activity,the MRSA300224h 1/2MIC after the effect of gallnut extract, beta lactam enzyme activity inhibition compared with the control group there were significant differences (P<0.01),compared with the positive control group, the difference was not significant. Synergistic effect of gallnut extract and gentamicin can significantly reduce the MIC of MRSA3002. Conclusion Gallnut extract can reduce β-lactamase activity recovery sensitivity of drug-resistant bacteria.
