Determination of content and encapsulation efficiency of leuprolideacetate microspheres
10.3969/j.issn.1005-1678.2017.01.014
- VernacularTitle:醋酸亮丙瑞林缓释微球药物含量及包封率的测定
- Author:
Hui TAO
;
Bo WANG
;
Song ZENG
;
Limei HE
;
Qing HUANG
;
Huaqing LIN
;
Rong LIU
- Keywords:
leuprolide acetate;
microspheres;
HPLC;
encapsulation efficiency
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(1):60-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a determination method for leuprolide acetate microspheres. Methods HPLC was performed on Inertsil ODS-SP (150×4.6 mm×5μm) with mobile phase consist of 0.1 mol/L of Ammonium Dihydrogen Phosphate(adjust its pH to 7.00±0.05 with Ammonium Hydroxide) and Acetonitrile in ratio of 3:1(V/V),and the flow rate was 1.0 mL/min.The wavelength was 220 nm and column temperature was 30℃. The injection volume was 20 μL. Results The linear range of leuprolide acetate was 20.0-160.0μg/mL (r=0.9999) with an average recovery of 99.80%,RSD=0.63%(n=9). Conclusion The method of HPLC was accurate,reliable and specific, which could be used to determinate the assay of leuprolide acetate microspheres and for quality control of microspheres.
