Protective Effect of Tibetan Medicine Compound Duoxuekang Capsules on Acute Lung Injury in Mice In-duced by Lipopolysaccharide
10.6039/j.issn.1001-0408.2017.07.18
- VernacularTitle:藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用
- Author:
Xiaoxin HU
;
Qiang LUO
;
Leilei DU
;
Yi ZHANG
;
Silang JIANGYONG
- Keywords:
Tibetan medicine;
Compound Duoxuekang capsules;
Acute lung injury;
Inflammation;
Oxidative stress;
Mice
- From:
China Pharmacy
2017;28(7):926-929
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effect of Tibetan medicine Compound Duoxuekang capsules on the acute lung injury in mice induced by lipopolysaccharide. METHODS:60 KM mice were randomly divided into blank group,model group,dexamethasone group (positive control,1 mg/kg) and Compound Duoxuekang high-dose,medium-dose,low-dose groups (3.6,1.8,0.9 g/kg,calculated by crude drug),10 in each group. Intragastrically administrated,mice in blank group and model group intragastrically administrated equal volume of normal saline,once a day. After 7 d of administration,except for blank group, mice in other groups intraperitoneally injected lipopolysaccharide to induce acute lung injury. After 6 h modeling,pathological changes in the lung tissue was observed,lung tissue water content was measured,superoxide dismutase(SOD),glutathione peroxi-dase(GSH-Px),malondialdehyde(MDA)levels in lung tissue and IL-6,TNF-α levels in serum were detected. RESULTS:Com-pared with blank group,mice in model group showed pathological changes in congestion and edema,inflammatory cell infiltra-tion,obvious widened alveolar septum and alveolar wall;water content in lung tissue and IL-6,TNF-αlevels in serum were signifi-cantly increased (P<0.01);SOD,GSH-Px levels in lung tissue were significantly decreased (P<0.01). Compared with model group,injury degree of lung tissue reduced to varying degrees in each treatment group,except for the water content,MDA level in lung tissue and TNF-α level in serum in Compound Duoxuekang capsules low-dose group;the above-mentioned indexes in other groups were significantly improved (P<0.05 or P<0.01). CONCLUSIONS:Compound Duoxuekang capsules can obviously re-duce mice's oxidative stress and inflammatory response,and has certain protective effect on acute lung injury in mice.
