Study on the Purification Technology of Sanhuang Yishen Formula
10.6039/j.issn.1001-0408.2017.07.27
- VernacularTitle:三黄益肾方的纯化工艺研究
- Author:
Xiaobin WU
;
Luolin WANG
;
Jiehuan LI
;
Rufan ZHONG
;
Liuping YUAN
;
Ming GUO
- Keywords:
Sanhuang yishen formula;
Purification technology;
Orthogonal test;
Astragaloside;
Total polysaccharide;
Calyco-sin glucoside;
Chitosan;
Clarifying agent
- From:
China Pharmacy
2017;28(7):957-960
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the purification technology of Sanhuang yishen formula. METHODS:Using retention rate and impurity rate of purified total polysaccharide,astragaloside and calycosin glucoside as index,the purification effects of water extraction and alcohol precipitation method (50%,60%,70% ethanol) and clarifying agent method (101 juice clarifying agent, ZTC natural clarifying agent,chitosan clarifying agent) were respectively detected to screen the purification method;orthogonal test was used to optimize the technology parameters(mass concentration of liquid,amount of clarifying agent and pH of liquid)by the optimized purification method,and the verification test was conducted. RESULTS:The purification was better when using chito-san as clarifying agent with comprehensive score of 98.62;the purified technology parameters were mass concentration of liquid 1 g/mL,1% chitosan solution amount of 2 mL/g,pH 5.1;the average value of retention rate and impurity rate of purified total poly-saccharide, astragaloside and calycosin glucoside in verification test were 79.56%(RSD=1.24%, n=3), 78.11%(RSD=0.97%,n=3),79.46%(RSD=1.03%,n=3)and 32.18%(RSD=1.16%,n=3),respectively. CONCLUSIONS:Using chito-san as clarifying agent shows good purification effect for Sanhuang yishen formula,which is simple. The optimized technology is stable and feasible.
