Content Determination of Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Wenyang Zhenshuai Granules by HPLC
10.3969/j.issn.1005-5304.2017.04.019
- VernacularTitle:HPLC测定温阳振衰颗粒中人参皂苷Rg1、 人参皂苷Re、人参皂苷Rb1含量
- Author:
Lei YANG
;
Manhua ZHANG
;
Huzhi CAI
;
Jilian SHI
- Keywords:
Wenyang Zhenshuai Granules;
ginsenoside Rg1;
ginsenoside Re;
ginsenoside Rb1;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(4):75-78
- CountryChina
- Language:Chinese
-
Abstract:
s: Objective To establish an HPLC method to determine the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Wenyang Zhenshuai Granules.Methods The separation was preformed on Agilent TC-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitril-0.05% aqueous solution of phosphoric acid with gradient elution (0–35 min, 19% acetonitrile; 35–58 min, 19%–29% acetonitrile; 58–70 min, 28% acetonitrile; 70–100 min, 29%–40% acetonitrile). The flow rate was 1.0 mL/min; the wavelength of detector was 203 nm; the temperature of column was 35℃.Results The calibration curves showed good linearity in the range of 0.22–2.2 μg (ginsenoside Rg1,r=0.9998), 0.22–2.2 μg (ginsenoside Re,r=0.9998) and 0.26–2.6 μg (ginsenoside Rb1,r=0.9991), respectively. The average recoveries of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 98.04%, 96.58% and 96.75%, respectively.Conclution The method is accurate, hypersensitized and reproducible, which can be applied to the quality control of Wenyang Zhenshuai Granules.
