Simultaneous Determination of Four Chemical Components in Carthamus tinctorius L. by HPLC
10.3969/j.issn.1005-5304.2017.04.020
- VernacularTitle:HPLC同时测定红花药材中4种化学成分含量
- Author:
Ting HE
;
Keao LI
;
Zhihong JI
;
Shuge TIAN
- Keywords:
Carthamus tinctorius L.;
hydroxysafflor yellow A;
rutin;
quercetin;
kaempferol;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(4):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the simultaneous determination of hydroxysafflor yellow A, rutin, quercetin, and kaempferol in Carthamus tinctorius L..Methods The Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) was used with diode array detection. The mobile phase was 0.5% phosphoric acid (A) and methanol (B) to separate the four components by gradient elution at 30℃; the flow rate was 0.8 mL/min; the injection Volume was 10 μL; the detection wavelength was at 360 nm.Results The linear ranges of hydroxysafflor yellow A, rutin, quercetin, and kaempferol were 0.0776–0.7760 μg (r=0.9999), 0.0460–0.4600 μg (r=0.9996), 0.0064–0.0640 μg (r=0.9999) and 0.0128–0.1280 μg (r=0.9997), respectively. The average recoveries of four components were 99.68% with RSD=2.29% (n=6), 99.78% with RSD=1.52% (n=6), 102.03% with RSD=1.02% (n=6), 97.03% with RSD=2.05% (n=6), respectively.Conclusion The method is convenient, accurate, sensitive, stable and repeatable, which can be a method for quality control of Carthamus tinctorius L..
