Comparative Stndy on Concentration Determination of Valproic Acid in Human Serum by LC-MS/MS and EMIT
10.6039/j.issn.1001-0408.2017.08.11
- VernacularTitle:LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究
- Author:
Hailang SHI
;
Haidong WANG
;
Xingfa SONG
- Keywords:
Valproic acid;
Serum concentration;
LC-MS/MS;
EMIT;
Difference
- From:
China Pharmacy
2017;28(8):1049-1052
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the difference in the concentration determination of valproie acid (VPA) in human serum by LC-MS/MS and EMIT.METHODS:Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients.The paired t-test,Pearson correlation analysis,Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination.RESULTS:The results of LC-MS/MS method was pos itively correlated with that of EMIT method (r=0.924,P＜0.05);the regression equation of them was cEMIT=0.920 7cLC.MS/MS-1.114 4 (r=0.924).Average serum concentrations of VPA determined by LC-MS/MS and EMIT were (49.9 ± 21.2) and (54.9 ± 21.3) μg/mL,with statistical significance (P＜0.05).The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS 8.3 μg/mL,95％ confidence interval was (-13.6,18.7).CONCLUSIONS:The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation.But the determination results still have certain difference,it is suggested to use same method for long term monitering.
