Determination of Ephedrine Hydrochloride in Tongxuan Lifei Pills by Accelerated Solvent Extraction Com-bined with QuEChERS Purification
- VernacularTitle:快速溶剂萃取联合QuEChERS净化法测定通宣理肺丸中盐酸麻黄碱的方法优选
- Author:
Huiyan LIU
;
Cuiling CHEN
- Keywords:
Accelerated solvent extraction;
QuEChERS purification;
Tongxuan Lifei pills;
Ephedrine hydrochloride;
Orthogonal design
- From:
China Pharmacist
2017;20(3):562-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the feasibility of the determination of ephedrine hydrochloride in Tongxuan Lifei pills by acceler-ated solvent extraction ( ASE) combined with QuEChERS purification, and compare the results with those of the extraction method in Chinese Pharmacopoeia. Methods: The extraction efficiency of ephedrine hydrochloride in Tongxuan Lifei pills determined by HPLC was used as the evaluation index, and the operation parameters of ASE were optimized by orthogonal experiments. Results: The opti-mum conditions of ASE for the determination of ephedrine hydrochloride in Tongxuan Lifei pills were as follows:the samples were defat-ted by n-hexane ( the extraction temperature was 80℃, the static extraction time was 5 min for one cycle, and the flush volume was 100%. ) ,and then methanol was used as the extraction solvent for the extraction of ephedrine hydrochloride ( the extraction temperature was 80℃,the static extraction time was 8 min for three cycles) ,in the end, the impurities were purified by PSA purifying agent. Using the optimized ASE method to extract ephedrine hydrochloride in Tongxuan Lifei pills, the extraction time was reduced to 40 minutes with less interference, and compared with that of the method in Chinese Pharmacopoeia, the determination of the relative standard devi-ation was less than 5%. Conclusion:The ASE technique combined with QuEChERS purification is simple, quick and effective,and it can be used as the pretreatment method for the determination of ephedrine hydrochloride in Tongxuan Lifei pills.
