Determination of Aflatoxin B1 in Edible Vegetable Oil by HPLC with Post-column Derivation
- VernacularTitle:柱后衍生高效液相色谱法测定食用植物油中的黄曲霉素B1的含量
- Author:
Wenyan FAN
;
Songwen SONG
;
Huanwei YANG
- Keywords:
Aflatoxin B1;
Thin layer fluorescence;
Immuno-affinity column;
Post-column derivation;
HPLC
- From:
China Pharmacist
2017;20(3):591-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method with post-column derivation for the determination of aflatoxin B1 in edible vegetable oil. Methods:An advanced biotechnology-immunoaffinity column was used for the extraction of aflatoxin Bl from the samples, and an HPLC method with post-column derivation was applied to detect aflatoxin Bl in edible vegetable oil, and the results were com-pared with those of the national standard thin layer fluorescence method. Results:The linear range of aflatoxin Bl was 10. 2-51. 0 ng · ml-1(r=0. 9996), the average recovery was 87. 3%(RSD=0. 96%, n=6), and the detection limit was 1 μg · kg-1. Conclu-sion:The method is simple, rapid and sensitive, which can be used as a promoted conventional method for the detection of a large number of samples.
