Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats
10.3969/j.issn.1006-9771.2017.03.010
- VernacularTitle:次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响
- Author:
Li CAI
;
Jin WANG
;
Yao CUI
;
Ge ZHANG
;
Songyan WU
;
Wendong ZHANG
;
Zhaohui LIU
- Publication Type:Journal Article
- Keywords:
infrasound;
calmodulin-dependent protein kinase II;
tau protein;
learning and memory;
hippocampus;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2017;23(3):298-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of infrasound on expression of calmodulin-dependent protein kinase II (CaMKII) and tau pro-tein in hippocampus of rats. Methods Fifty-six male Sprague-Dawley rats were randomized into control group (n=8), 1-day group (n=8), 7-day group (n=8) and 14-day group (n=32), and the 14-day group was subgrouped as 1-hour, 6-hour, 24-hour, 48-hour subgroups, naming after the time after infrasound exposure, 8 in each subgroup. All the test groups were put in an infrasound field with 8 Hz, 130 dB for 2 hours daily, while the control group was put in the infrasound instrument without infrasound exposure for 2 hours daily. The expression of pT286-CaMKII and tau protein in hippocampus was detected with immunohistochemisty, Western blotting and enzyme-linked immunoabsor-bent assay. Results The expression of pT286-CaMKII was the most in 14-day group (F>14.912, P<0.001), as well as the expression of tau pro-tein (F>36.229, P<0.001), and secondary in 7-day group (P<0.05). For 14-day group, the expression of tau protein was the most in 1-hour and 6-hour subgroups, and dropped down in 24-hour subgroup, although more than that in the control group (P<0.05). Conclusion Exposure of 8 Hz, 130 dB infrasound may induce phosphorylation of CaMKII and tau protein, and the expression of tau protein in hippocampal cells in rat, which may disturb their learning and memory function.
