Determination of 7 Kinds of Ginsenoside in Qipi Pills by HPLC-QTOF-MS
- VernacularTitle:高效液相色谱四级杆飞行时间串联质谱法测定启脾丸中7种人参皂苷的含量
- Author:
Hongyan CHEN
;
Chenxu YAO
;
Xiaolong ZHANG
- Keywords:
HPLC-QTOF-MS;
Qipi pills;
Ginsenoside;
Quantitative determination;
Method validation
- From:
China Pharmacist
2017;20(3):460-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To simultaneously determine the contents of ginsenoside Rb1 , ginsenoside Rb2 , ginsenoside Rb3 , ginsen-oside Re, ginsenoside Rg1 , ginsenoside Rf and ginsenoside Ro in Qipi pills by HPLC-QTOF-MS. Methods: The determination was performed on an Agilent Poroshell 120 EC-C18 column (2. 1 mm × 50 mm,2. 7 mm) with mobile phase consisting of acetonitrile( A)-water(B, containing 0. 1% formic acid) with gradient elution. The flow rate was 0. 21 ml·min-1. The column temperature was 30℃. The MS instrument was equipped with an ESI+ ion source. The exacted ion chromatograms were used to determine the quantities of different compounds in the samples while the mass spectra of product ions were used for confirming the compounds. Results:All the 7 kinds of ginsenoside showed good linearity (r>0. 9993). The RSDs of precision, repeatability and stability tests were all less than 5%. The average recoveries were within the range of 97. 11%-101. 98%. Conclusion:The method is simple, rapid and reliable with high specificity, which can be used for the quality control of Qipi pills.
