Study on the HPLC Fingerprint of Gynecological Lotion
- VernacularTitle:妇科洗剂的HPLC指纹图谱研究
- Author:
Liu YANG
;
Ting LI
;
Yanbo FAN
;
Zheren LIN
;
Yisheng ZHANG
- Keywords:
Gynecological lotion;
HPLC;
Fingerprint;
Similarity evaluation
- From:
China Pharmacist
2017;20(3):463-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint detection method for gynecological lotion. Methods:Wondasil C18 (250 mm × 4. 6 mm,5 μm)was selected as the analytical column. The mobile phase was composed of acetonitrile-0. 3% phosphric acid and 0. 3% diethylamine solution with gradient elution at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 284 nm and the column temperature was 30℃. Ten batches of gynecological lotion were detected by the HPLC fingerprint and evaluated by Chromato-graphic Fingerprint Evaluation System of Chinese Medicine (2004 edition). Results: The separated peaks were clear and 15 common peaks were identified in the fingerprint of 10 batches of gynecological lotion. Conclusion: The HPLC fingerprint is with good repeat-ability and stability, which can provide evidence for the quality control of gynecological lotion.
