Extraction Conditions Optimization and Content Determination of Gallic Acid in Wudang Pineneedle Tea by Orthogonal Test
- VernacularTitle:"正交试验优化""武当松针茶""中没食子酸提取条件及含量测定"
- Author:
Cong LI
;
Peng LI
;
Fang ZHENG
;
Xuesong ZHU
;
Fangjian YE
- Keywords:
Wudang pineneedle tea;
Gallic acid;
Orthogonal test;
Assay;
HPLC
- From:
China Pharmacist
2017;20(3):476-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction conditions of gallic acid inWudang pineneedle tea and establish the assay meth-od. Methods:The content of gallic acid was determined by RP-HPLC-DAD. Orthogonal test was used to optimize the extraction condi-tions of gallic acid in Wudang pineneedle tea on the base of single factor tests. The optimal extraction conditions were as follows:adding 50-fold amount of water, and ultrasonic extracting for 60 min under 200W ultrasound power. Results:The linear range of gallic acid was 5. 10-51. 00 mg ·L-1(r=0. 9993), and the average recovery was 98. 7% (RSD=1. 93%, n=6). The average content of gallic acid in the samples was 0. 35 mg·g-1 . Conclusion: The optimal extraction conditions are simple and feasible, and the assay method is stable and reliable, which can be used for the extraction and content determination of gallic acid in Wudang pineneedle tea.
