Simultaneous Determination of Morphine and Codeine Phosphate in Compound Liquorice Tablets by HPLC-ESI-MSMS
- VernacularTitle:液相色谱电喷雾串联质谱同时测定复方甘草片中吗啡与磷酸可待因
- Author:
Lu GAO
;
Jialin WANG
;
Xiujuan XIE
- Keywords:
Compound liquorice tablets;
Morphine;
Codeine Phosphate;
HPLC-ESI-MSMS
- From:
China Pharmacist
2017;20(3):482-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the content of morphine and codeine phosphate in compound liquorice tablets. Methods:The analysis was performed by HPLC-ESI-MSMS. The acquisition was MRM ( multi-reaction monitor) , the column wasAgilentZorbaxEclipseSBC18(2.1mm×100mm,3.5 μm),themobilephasewasacetonitrileand15mmol·L-1ammoniumac-etate with gradient elution. Results:The precursor (m/z) of morphine and codeine was 286. 0 and 300. 0, respectively, and the quan-titative ion was 165. 0 and 165. 0, respectively. The linearity of morphine was excellent between 25 and 500ng·ml-1 , and the average recovery was 103. 1%. The linearity of codeine was good between 0. 75 ng and 150 ng·ml-1 , and the average recovery was 101. 5%with RSD of 1. 7(n=6). Conclusion:The method is simple and accurate, which can be used for the quality control of compound ligu-orice tablets.
