Study on Quality Standard for Shengji Ointment
- VernacularTitle:生肌软膏质量标准的建立
- Author:
Da TONG
;
Jing QIU
;
Ruilian LI
- Keywords:
Shengji ointment;
Quality standard;
TLC;
HPLC;
Silver sulfadiazine
- From:
China Pharmacist
2017;20(3):565-567
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the quality standard for Shengji ointment. Methods:A TLC method was used to identify Resina draconis and Olibanum. An HPLC method was used to determine the content of silver sulfadiazine. The detection wavelength was set at 254 nm. The determination was performed on a Wondasil C18 column(250 mm × 4. 6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0. 1% phosphoric acid(8: 92). The injection volume was 5 μl. Results:The TLC spots were clear, specific and repro-ducible. A good linear relationship was obtained between the peak areas and the concentrations of sulfadiazine within the range of 40. 97-1024. 18 μg·ml-1(r=0. 9999). The average recovery was 100. 17% (RSD=0. 64%,n=6). Conclusion: The methods are easy-operated, specific, reproducible and accurate, which can be used for the quality control of the preparation.
