LncRNAs expression profile of ankylosing sporidylitis revealed by microarray
10.3760/cma.j.issn.1008-1372.2017.02.013
- VernacularTitle:长链非编码RNA在强直性脊柱炎患者中的差异表达研究
- Author:
Xiaowu ZHONG
;
Chengjiao YAO
;
Yufeng QING
;
Yaxi YAO
;
Yonglong HE
;
Qibin YANG
;
Mingcai ZHAO
;
Wenguang XIE
;
Jingguo ZHOU
- Keywords:
RNA/ME;
Spondylitis,ankylosing/ME;
Microchip analytical procedures
- From:
Journal of Chinese Physician
2017;19(2):214-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression profile variation of long non-coding RNAs (lncRNAs) in ankylosing sporidylitis (AS) and explore the role of lncRNAs in the pathogenesis of AS.Methods The peripheral blood mononuclear cells of AS patients and health controls (HC) were used to detect for differently expressed lncRNAs by microarray.The roles of lncRNAs were predicted with GO and pathway analysis.The results were verified by real time-polymerase chain reaction (PCR).Results A total of 148 lncRNAs and 134 mRNAs were detected,which had more than 2-fold differentially expressed in AS patients.Bioinformatics analysis found that GO term enrichment included protein binding,regulation of transcription,metabolism,signal transduction,et al.and might involve in toll-like receptor pathway,protein kinase,complement pathway,notch signaling pathway and so on.The expressions of three lncRNAs were estimated by real time-PCR which found that consistent with that of microarrays.Among these,D90064 was the most aberrantly expressed lncRNAs.Conclusions Several lncRNAs expression was changed significantly in AS patients in comparison with HC,which implies that those different lncRNAs may have an important role in the development and progression of AS.
