A stable method to label two different types of retinal ganglion cells in mouse retina
10.13389/j.cnki.rao.2017.0030
- VernacularTitle:一种稳定标记小鼠视网膜内两种不同类型神经节细胞的方法
- Author:
Songtao WANG
;
Honglei XIAO
;
Guomin ZHOU
- Keywords:
mouse;
retina;
retinal ganglion cell;
intrinsically photosensitive retinal ganglion cells
- From:
Recent Advances in Ophthalmology
2017;37(2):114-116
- CountryChina
- Language:Chinese
-
Abstract:
ObJeetive To report a simple and efficient method to label two different types of retinal ganglion cells (RGCs) in mouse retina.Methods Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal nmicroscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglion cells (ipRGCs) using Brn3a and Melanopsin.Results The double staining results of whole mount retina showed that conventional RGCs and melanopsin immunopositive ipRGCs had a complementary distribution in mouse retina,these two subtypes of RGCs were predominantly present in the ganglion cell layer.The numbers of ipRGCs was just about 1％-2％ of conventional RGCs,and the axons of ipRGCs toward the direction of the optic disc,several dendrites toward the inner plaximem layer.Conclusion The immunofluorescence double staining of whole mount retina is a simple,stable and efficient method to label two different types of mouse RGCs.
