TIMP-1 and Ang-1 gene-modified BMSCs transplantation to improve cardiac function of rats with myocardial infarction
10.3760/cma.j.issn.0254-1785.2016.09.009
- VernacularTitle:TIMP-1和Ang-1基因修饰的BMSC输注对心肌梗死后大鼠心功能的影响
- Author:
Jie LI
;
Hua WU
;
Dawei LI
;
Haoran WANG
;
Shulin JIANG
- Keywords:
Mesenchymal stem cell transplantation;
Genetically Modified;
Tissue inhibitor of metalloproteinases 1;
Angiopoietin 1;
Myocardial infarction;
Therapy;
Rats
- From:
Chinese Journal of Organ Transplantation
2016;37(9):556-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of TIMP-1 and Ang-1 gene-modified BMSCs transplantation on the left ventricular function of rats with myocardial infarction.Methods The rat BMSCs were.transfected with eukaryotic expression plasmid encoding TIMP-1 or/and Ang-1 gene by liposome.Acute myocardial infarction was made in male rats by ligation of the left anterior descending (LAD) coronary artery.BMSCs carrying TIMP-1 or/and Ang-1 gene were injected into the ischemic myocardium after LAD ligatior.Four weeks after the administration,cardiac function was assessed by echocardiography and the hearts were harvested and sectioned for immunohistochemistry to examine the apoptosis,the collagen content and angiogenesis density.Results TIMP-1 and Ang-1 genemodified BMSCs transplantation significantly improved the cardiac function,myocardial apoptosis was alleviated,collagen content decreased and the angiogenesis density in border-zone was increased significantly (P＜0.05).Conclusions The results suggest that the combination of TIMP-1 and Ang-1-gene modified BMSCs transplantation can improve the cardiac function of rats with myocardial infarction.The increase of the blood supply,the alleviation of myocardial apoptosis and ventricle remolding after myocardial infarction possibly play important roles in the mechanism.
