Establishment of a mouse model of kidney deficiency induced by oral administration of hydrocortisone and evaluation of related factors
10.3969/j.issn.1005-4847.2017.01.013
- VernacularTitle:氢化可的松致肾虚证小鼠模型的建立及相关指标的评价
- Author:
Bing DAI
;
Jiani ZHANG
;
Menglin YANG
;
Yuxing LI
;
Zizeng XIAO
;
Lei YANG
;
Qiang WEI
- Keywords:
Kidney-yang deficiency;
Kidney?yin deficiency;
Evaluating factors;
Mouse models
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(1):70-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model of kidney-yin and kidney?yang deficiency after oral administra?tion of hydrocortisone, and to explore the related evaluation factors. Methods The model was established by oral adminis?tration of hydrocortisone to induce kidney?yin and kidney?yang deficiency in mice. The survival and body weight of the mice were observed. The serum content of adrenal cortical hormone ( ACTH) , cortisol ( Cor. ) in the hypothalamic?pituitary?ad?renal (HPA) axis, and thyroid stimulating hormone (TSH), triiodothyronine (T3), thyrox (T4) in the hypothalamic?pi?tuitary?thyroid (HPT) axis, follicle?stimulating hormone (FSH), estradiol (E2), testosterone (T) in the hypothalamic?pituitary?gonadal ( HPG) axis were determined by radioimmunoassay. Results The body weight of kidney?yin and kidney?yang mice were decreased, the serum ACTH, Cor, TSH, T3, T4 contents were decreased, the serum FSH, E2, T contents were increased in the kidney?yang deficiency model mce ( P<0. 01 ) , and those parameters in the kidney?yin deficiency model mice were changed in opposite direction. Conclusions It is found that the hormone levels of ACTH, Cor, TSH, T3, T4, FSH, E2 and T in kidney deficiency mice are changed, and cortisol can be used as an important index to evaluate the model of kidney deficiency induced by glucocorticoid.
