Change of Caspase-3 and PSD-95 Expression in Hippocampus of Mice with Chronic Ketamine Addiction

VernacularTitle:氯胺酮长期滥用后小鼠海马区caspase-3和PSD-95的表达变化
Author: Ao DU ; Peng REN ; Bolin HE ; Shuncheng YANG ; Runtao DING ; Ruipeng SHEN ; Yanning LI ; Zhibin DONG ; Yan LU ; Xu WU
Keywords: ketamine; caspase-3; postsynaptic density 95; hippocampus
From: Journal of China Medical University 2017;46(3):193-196
CountryChina
Language:Chinese
Abstract: Objective To study the changes of hippocampal caspase-3 and PSD-95 expression levels in the mice exposed to ketamine 30 mg/(kg·d)for three months. Methods Forty C57BL/6 mice were randomly divided into two groups,and the chronic ketamine addiction model was established by giving mice a three month course of daily intraperitoneal injections of ketamine. Immunohistochemical study and Western blot-ting were applied to observe the expression of caspase-3 and PSD-95 protein. Results There were more expression of caspase-3 and less of PSD-95 in ketamine group as detected by immuohistochemistry. Western blotting results showed caspase-3 active fragment level significantly increased com-pared to saline group,but PSD-95 protein level was decreased. Conclusion The increased level of caspase-3 protein and reduced expression of PSD-95 are observed after long-term ketamine administration. These findings may provide an evidence for the neurotoxicity in mouse hippocampus of chronic ketamine addition as a recreational drug.