The effect and mechanism of GGNBP2 regulates the proliferation, invasion and migration of human U251 glioma cells
10.3969/j.issn.1002-0152.2016.12.002
- VernacularTitle:锌指蛋白403对人U251胶质瘤细胞增殖、侵袭和迁移的影响
- Author:
Jia WANG
;
Xi HU
;
Peng YUAN
;
Yongbing DENG
- Keywords:
GGNBP2;
Glioma;
Proliferation;
Invasion;
Migration
- From:
Chinese Journal of Nervous and Mental Diseases
2016;42(12):710-714
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of GGNBP2 on proliferation,migration and invasion of human gli-oma U251 cells and the potential mechanism. Methods U251 glioma cells were transfected with lentiviral vector carrying GGNBP2 to establish a stable overexperssion of GGNBP2 in U251 cell line. After verification of the efficiency of transfec-tion by real-time PCR, Western Blot, PCR and CCK-8 were applied to detect the proliferation of U251 cells. The Tran-swell chamber assay was applied to measure the migration and invasion of human U251 glioma cells. Western blot was applied to measure protein levels of AKT, p-AKT, PCNA and MMP9. Result The stable U251 glioma cell line overex-pressing GGNBP2 was successfully established. Compared with the control group, overexpression of GGNBP2 significant-ly inhibited the proliferation(P<0.05),invasion(57±6 vs. 203±6,205±7,F=512.4,P < 0.05)and migration (74±7 vs. 254±14,248±13,F=242.5,P<0.05) of U251 cells. The protein expression levels of p-AKT (F=45.4, P<0.05), PCNA (F=348.5, P<0.05) and MMP-9 (F=88.7, P<0.05) in GGNBP2 group were markedly decreased compared with the control group. Conclusion GGNBP2 may suppress the proliferation, invasion and migration of glioma though down-regulation of p-AKT, which in turn inhibits PCNA and MMP-9 expression.
