Comparative?analysis?of?two?different?DNA?purification?methods?for?bones?and?teeth
10.13618/j.issn.1001-5728.2017.01.015
- VernacularTitle:两种骨骼及牙齿纯化方法的比较分析
- Author:
Wenyan REN
;
Wei SHEN
;
Weiwei WU
;
Honglei HAO
;
Yanjia SU
- Keywords:
forensic biological evidence;
bones and teeth;
silica-extraction method;
silico membrane based method
- From:
Chinese Journal of Forensic Medicine
2017;32(1):55-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of silica-extraction method and Silico membrane based method in DNA purification from bones and teeth.Methods DNA samples were purified respectively with the silica-extraction method and MinElute PCR Purification kit from 6 bones and 8 teeth,then tested STR types by GlobalFiler? kits. And evaluated the two methods with the success rate and the peak height. Results Both of the two purification methods can successfully obtain the STR markers of the 14 samples. And there was no statistical difference between the two methods in the average peak height from bones and teeth. Conclusion The Silico membrane based method which have more advantages in operation is an efficient method to purify DNA from bones and teeth, and there is no significant difference compared with the silica-extraction method. But the cost is higher. It can be selectively used in forensic practice.
