The Apoptosis Effect of Docetaxel Combined with Gamma Knife on Hepatoma Cells Xenograft in Nude Mice
- VernacularTitle:多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响
- Author:
Jingxian LI
;
Mi HE
;
Rui LIU
;
Yiyuan XIN
;
Yuanxu JIAN
;
Jiaping WANG
;
Ruimin ZHAO
- Keywords:
Docetaxel;
Gamma knife;
Xenograft in nude mice;
Apoptotic index
- From:
Journal of Kunming Medical University
2016;37(11):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the apoptosis effect of docetaxel combined gamma knife on hepatoma cell SMMC-7721 subcutaneous xenograft in nude mice.Methods Subcutaneous xenogyaft models were constructed and were divided into two groups:control group and experimental group.The experimental group was treated with docetaxel 60ug/0.3ml once every 3 days for 6 times and gamma irradiation once every other day for 6 times (with indoor temperature of 137Cs radiation source irradiating the tumor and of fractionated schedule 5Gy with the total dose of 10Gy every time).The control group was treated with physiological saline with the same dose of 60 ug/0.3 mL.Tumor growth was observed.Tumor samples were cut 30 days after the treatment and TUNEL was used to detect the apoptosis of tumor cells.Results Tumor growth rate in experimental group significantly slowed down.Apoptotic index in experimental groups was significantly higher than that in control group (P ＜ 0.05) Cornclusion Docetaxel combined gamma knife can inhibit the growth of hepatocellular carcinoma in nude mice.
