Detection on expression levels of mazE F toxin-antitoxin system in Mycobacterium tuberculosis by qRT-PCR
10.3969/j.issn.1002-2694.2017.02.010
- VernacularTitle:qRT-PCR检测结核分枝杆菌毒素-抗毒素系统mazE F的表达
- Author:
Wei LIU
;
Jili ZHAO
;
Yanlin QU
;
Wanying XIE
;
Li YUAN
- Keywords:
Mycobacterium tuberculosis;
toxin-antitoxin system;
drug resistance;
multidrug resistance
- From:
Chinese Journal of Zoonoses
2017;33(2):143-147
- CountryChina
- Language:Chinese
-
Abstract:
We investigate the different expression of toxin gene mazF3,6,9 and antitoxin gene mazE3,6,9 in the drug-resistance Mycobacterium tuberculosis,we used quantitative real-time polymerase chin reaction method to detect the expression level of toxin gene mazF3,6,9 and antitoxin gene mazE3,6,9 in M.tuberculosis (20 mono-resistance strains,20 multidrug resistance strains and standard strain H37Rv).The differences of gene expression levels between groups were analyzed by oneway ANOVA.Contrasting with control group,toxin genes mazF6,9 were up-regulated expression levels both in mono-resistance (11.1519±22.31721;8.4306±17.97897) and multidrug resistance (4.6016±1.29018;6.9627±6.92948),had statistical significance (P＜0.01),mazF3 expression levels had statistical significance neither in mono-resistance nor in multidrug resistance (P＞0.05);antitoxin genes mazE3 was in down-expression level,and had statistical significance both in mono-resistance (0.3606±0.12527) and multidrug resistance (0.2016±0.16542) (P＜0.01),mazE6 had no statistical significance (P＞0.05)either in mono-resistance or multi drug resistance,mazE9 only in multidrug resistance(0.3989±0.37679) was in downexpression level,and has statistical significance (P＜0.001).The toxin gene mazF6,9 and antitoxin gene mazE3,9 may participate in the drug-resistance formation of M.tuberculosis.
