Research on anti-cervical cancer effect of araloside mediated by NF-kappa B signaling pathway and its mechanism
10.3969/j.issn.1671-8348.2017.07.008
- VernacularTitle:NF-κB信号通路介导楤木皂甙抗宫颈癌作用及机制研究
- Author:
Haixia DUAN
;
Donghong LI
;
Lei BAO
;
Huan LI
;
Hongmin GAO
- Keywords:
uterine cervical neoplasms;
araloside;
autophagy;
NF-kappa B
- From:
Chongqing Medicine
2017;46(7):889-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibition role of araloside on cervical cancer HeLa cell proliferation and migration to reveal the molecular mechanism of NF-κB in HeLa cell in the process of tumor suppression.Methods The in vitro cultured cervical cancer HeLa cell line served as the research object.The experiment was divided into the control group and araloside(200 μg/mL) treatment group(observation group).The change of proliferation and migration ability after 48 h were observed in the two groups.Western blot was used to detect the expression of NF-κB molecule and change of autophagy involved protein Beclin 1 and LC3B.Results Araloside could significantly inhibit the proliferation and migration of HeLa cells and the NF-κB signaling pathway,and promoted the expression of autophagy related molecule Beclin 1,Atg 5 and LC3B.Conclusion Araloside could inhibit the NF-κB signaling pathways,thus induces autophagy occurrence and influences the proliferation and migration of cervical cancer HeLa cells.
