Effects of astragaloside on proliferation and apoptosis of keloid fibroblasts
10.3969/j.issn.1671-8348.2017.06.008
- VernacularTitle:黄芪总苷液对瘢痕疙瘩成纤维细胞增殖凋亡的影响
- Author:
Yu FU
;
Liang ZHANG
;
Na CHEN
;
Zhi YAN
;
Jing YANG
- Keywords:
astragaloside;
keloid;
fibroblasts;
survivin;
p53
- From:
Chongqing Medicine
2017;46(6):746-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of astragaloside on proliferation and apoptosis in human keloid fibroblasts.Methods The human keloid fibroblast ceils were treated with different concentration of astragaloside(10、20、40 ng/mL).Cell proliferation was detected by MTT,the gene expreesion levels and protein levels of apoptosis-related proteins,survivin,p53 and Bcl-2.were determined by real-time PCR and Western blot,respectively.Results Comparecl with control group(treated with 0 ng/mL astragaloside),the absorbance values (A490 nm) of each concentration group were significantly reduced,which suggest that the proliferation of all keloid fibroblast were markably inhibited in a dose-dependent way (P＜0.05).The gene expreesion levels and protein levels of apoptosis-related proteins,survivin、Bcl-2 were largely suppressed and P53 werelargely promoted in a dose-dependent.Conclusion The keloid fibroblasts cells proliferation and apoptosis could be regulated by astragaloside.
