Role of NFIC on cAMP-mediated diferentiation of stem cells from the apical papilla

VernacularTitle:转录因子NFIC在cAMP信号通路调控根尖牙乳头干细胞分化中的作用
Author: Yan LIANG ; Jing ZHANG ; Song LI
Keywords: stem cells from the apical papilla; cAMP; NFIC; differentiation
From: Acta Universitatis Medicinalis Anhui 2017;52(2):190-193
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of NFIC on the stimulation effects of cAMP-induced differentiation of stem cells from the apical papilla ( SCAPs) in vitro. Methods SCAPs isolated from dental papilla of human imma-ture third molars were cultured by enzyme digestion. SCAPs were transfected with lentivirus that overexpressed NF-IC gene ( ov-NFIC) or an empty vector ( LV-empty) and co-treatment with Forskolin. Mineralized nodule formation of each group was measured by alizarin red staining. Quantitative real-time reverse-transcription polymerase chain reaction was performed to test the expressions of RUNX2,ALP,OCN mRNA. Results Forskolin increased the ex-pression of Runx2, ALP, OCN mRNA as well as matrix mineralization in SCAPs, and the stimulation effects of For-skolin were enhanced by overexpressing NFIC gene. Conclusion The results indicate that NFIC can promote cAMP-induced differentiation of SCAPs.