Studies on the effect of ulinastatin on hippocampal neuron and MMP-9 expression after ischemia-reperfusion injury rats

VernacularTitle:乌司他丁对脑缺血再灌注损伤大鼠海马神经元形态及MMP-9表达的作用研究
Author: Lijun CAO ; Zemeng ZHU ; Jin WANG
Keywords: Ulinastatin; reperfusion injury; hippocampus; matrix metalloproteinase-9.
From: Chinese Journal of Biochemical Pharmaceutics 2017;37(3):41-43
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of ulinastatin on hippocampal neuron and matrix metalloproteinase-9 ( MMP-9 ) expression after ischemia-reperfusion injury rats, to reveal the mechanism of protection on hippocampus of ulinastatin.Methods The focal ischemia reperfusion model was made by thread embolism of middle cerebra1 artery, and ulinastatin was used.The animals were killed at 24 h after reperfusion was started.The sections of the brains were processed with Nissl staining and by immunohistochemistry with antibody of MMP-9.Results Corrected optical density (COD) of MMP-9 expression was significantly decreased after administration of ulinastatin.Also ulinastatin can obviously protect the structure of hippocampal neuron.Conclusion Ulinastatin could protect pyramidal cells in CA1 region from damage of ischemia-reperfusion injury and increase the number of normal neurons.Inhibition on MMP-9 expression was involved in the mechanism.