Stady on the expression of miRNA-21 in renal tubular epithelial cells
10.3969/j.issn.1005-1678.2017.03.011
- VernacularTitle:纤维化肾小管上皮细胞miRNA-21表达情况研究
- Author:
Yu ZHONG
;
Wenlun HAN
;
Yunyun ZHU
;
Zaichun XU
- Keywords:
renal tubular epithelial cells;
renal fibrosis;
miRNA-21;
TGF-β1;
BMP-7
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(3):44-47
- CountryChina
- Language:Chinese
-
Abstract:
To describe the relationship between miRNA-21 and renal fibrosis.Methods NRK-52E cells cultured in vitro,then constructed fibrosis model induced by transforming growth factorβ1 ( TGF-β1 ) , then bone morphogenetic protein-7 ( BMP-7 )-containing lentivirus vector was used to transfect the NRK-52E cells to anti-fibrosis, SMAD1, SMAD6, FN1, rno-miR-21-5p gene expression were detected.Results After TGF-β1 treatment, the BMP-7 mRNA and protein expressions were significantly decreased, FN1 mRNA and protein expressions were significantly increased ( P<0.05 ) . After transfected by BMP-7-containing lentivirus vector, BMP-7 mRNA expression significantly increased(P<0.05), FN1, SMAD6 and rno-miRNA-21-5p mRNA decreased, SMAD1 mRNA increased(P<0.05).Conclusion Epithelial-to-mesenchymal transition (EMT) is positively correlated with the miRNA21 and TGF-beta 1, its maybe related to the changes of BMP-7, SMAD1 and SMAD6.
