Determination of Three Active Components in Shuanghua Caoshanhu Hanpian by HPLC
10.3870/j.issn.1004-0781.2017.02.018
- VernacularTitle:高效液相色谱法测定双花草珊瑚含片中3种活性成分的含量
- Author:
Dezhi TANG
- Keywords:
Shuanghua Caoshanhu hanpian;
Chlorogenic acid;
Isofraxidin;
Rosmarinic acid;
Chromatography,high performance liquid
- From:
Herald of Medicine
2017;36(2):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of three active components in Shuanghua Caoshanhu Hanpian.Methods Agilent TC-C18 (4.6 mm × 250 mm,5 μm) column was used.The mobile phase was acetonitrile-water containing 0.2% phosphoric acid with gradient elution mode at a flow rate of 1.0 mL· min-1,the column temperature was 30 ℃,the detection wavelength was 327 mn (0-10 min) for chlorogenic acid,342 nm (10-35 min) for isofraxidin and msmarinic acid.Results Chlorogenic acid had a good linearity in the range of 0.052-0.939 μg (r =0.999 8,n =6),the average recovery was 99.23% with RSD of 1.4% (n =9).Isofraxidin had a good linearity in the range of 0.004-0.077 μg (r =0.999 8,n =6),the average recovery was 100.33 % with RSD of 1.8 % (n =9).Rosmarinic acid had a good linearity in the range of 0.012-0.213 μg (r =0.999 9,n =6),the average recovery was 99.42% with RSD of 1.4% (n =9).Conclusion The method is simple,accurate and reliable.It can be used to determine chlorogenic acid,isofraxidin and rosmarinic acid in Shuanghua Caoshanhu hanpian at the same chromatogram condition.