Technology Optimization of Ultrasonic-enzyme-assisted Extraction of Sanguinarine from Zanthoxylum nitidum
10.3870/j.issn.1004-0781.2017.02.019
- VernacularTitle:超声-酶法提取两面针中血根碱的工艺优选
- Author:
Shihui LU
;
Ran CHEN
;
Yao LIN
;
Nan CHEN
;
Xiaoying ZHU
- Keywords:
Zanthoxylum nitidum;
Sanguinarine;
Ultrasonic-enzyme-assisted;
Extraction technology
- From:
Herald of Medicine
2017;36(2):193-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the technology conditions of ultrasonic-enzyme-assisted extraction for sanguinarine from Zanthoxylum nitidum.Methods Extraction rate of sanguinarine determined by HPLC was served as an index.The applicability of the extraction solvent added with acid and enzymatic hydrolysis pretreatment to the ultrasonic-enzyme-assisted extraction of Zanthoxylum nitidum was investigated.Ultrasonic power,extraction frequency and solvent volume were optimized by orthogonal experiment.Finally,ultrasonic extraction time was optimized in dynamic process.Results The optimal process was as follows:Zanthoxylum nitidum powder was extracted 3 times by ultrasonic-wave (250 W) with 40％ ethanol (0.2％hydrochloride) as solvent (extracted for 15 rmin with 6-fold solvent at the first time,then extracted for 12 min with 3-fold solvent at the second and the third time,respectively).The extraction rate of sanguinarine was 88.6％.Conclusion The process is economic,efficient,energy-and time-saving,and provides experimental basis for industrial production of sanguinarine.
