Comparison of HPLC and CMIA Method in Monitoring Concentration of Phenytoin in Epileptic Patients' Plasma
10.3870/j.issn.1004-0781.2017.02.004
- VernacularTitle:高效液相色谱法与化学发光微粒子免疫检测法测定血浆苯妥英浓度的一致性评价
- Author:
Jing LU
;
Yan CHEN
;
Meihua GUO
;
Zhao QIAN
;
Ting QU
;
Lijuan DUAN
;
Xin HAI
- Keywords:
Phenytoin;
Chromatography,high performance liquid;
Chemiluminescence microparticle immunoassay;
Plasma concentration;
Consistency
- From:
Herald of Medicine
2017;36(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography (HPLC) method for determining phenytoin concentration in epilepsy patients' plasma,and compare this method with chemiluminescence microparticle immunoassay (CMIA),and to evaluate the consistency of the two methods.Methods HPLC and CMIA methods were applied to determine the plasma concentration of phenytoin in 60 epileptic patients,respectively.The difference of results was analyzed by two-side paired t-test,and then the correlation and consistency of the two methods were investigated with Passing-Bablok regression and Bland-Altman method.Results There was no significant difference between the results of the two methods (P ＞0.05).The regression equation of the determination results by HPLC (Y) and CMIA (X) was Y=0.992 9X +0.143 7 (R2 =0.992 6,n =60),which indicated the correlation of the two methods was good.Bland-Altman analysis showed that the consistency of the two methods for determining was good.Conclusion HPLC and CMIA method in monitoring plasma concentration of phenytoin have good correlation and consistency.Both methods can be used for therapeutic drug monitoring of phenytoin.
