Effect of bushenyangxue prescription on the levels of anti-mullerian hormone in rat with premature ovarian failure

VernacularTitle:补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素的影响
Author: Xia JI ; Jinying FU ; Bingyu WANG ; Junpan HU
Keywords: Bushenyangxue prescription; Premature ovarian failure ( POF); AMH
From: Chinese Journal of Comparative Medicine 2017;27(1):49-53
CountryChina
Language:Chinese
Abstract: Objective Establish premature ovarian failure ( POF ) model in female Sprague Dawley by tripterygium wilfordii , and investigate the effect of bushenyangxue prescription on the levels of anti -mullerian hormone ( AMH) .Methods After POF model was established , rats were gave by gavage of different dosage of Bushenyangxue prescription for 30 days.The changes of histomorphology on rat ovarian tissue were observed by hematoxylin -eosin staining. Serum AMH concentraction , protein and mRNA expression of AMH were measure with ELISA , immunohistochemical staining and qRT-PCR, respectively .Results The follicle and corpus luteum were atrophied after tripterygium wilfordii challenge, which was improved after treatment with Bushenyangxue prescription .Serum AMH, protein and mRNA expression of AMH were decreased tripterygium wilfordii-treated rats; this decrease was inhibited after treatment with Bushenyangxue prescription .Conclusions Our study indicates that Bushenyangxue prescription could preserve the AMH levels of POF rats.These findings suggest that Bushenyangxue prescription may be a useful strategy to treat POF .