The modulation mechanism of LiCl inhibited the Pseudomonas aeruginosa-induced inflammation
10.3969/j.issn.1673-4130.2017.01.005
- VernacularTitle:氯化锂抑制铜绿假单胞菌感染引起的炎性反应调节机制研究
- Author:
Qiang FU
;
Kang CHEN
;
Fuda HUANG
;
Lishao MIAO
;
Shanhong YANG
;
Xiuming ZHANG
- Keywords:
LiCl;
pseudomonas aeruginosa;
inflammatory cytokine;
cell apoptosis
- From:
International Journal of Laboratory Medicine
2017;38(1):13-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of LiCl in modulating bacterial-mediated inflammation after Pseudomonas aeruginosa infection.Methods Western-blot was used to determine the efficacy of LiCl usage.The expression of inflammatory cytokines in Pseudomonas aeruginosa-infected macrophages and neutrophils was detected by qPCR.Cell apoptosis was measured by flow cytometry.Results Western-blot data showed that LiCl up-regulated the protein levels of p-GSK-3β(Ser 9)and β-catenin in macrophages and neutrophils,indicating the efficacy of LiCl usage.qPCR data indicated that LiCl enhanced the expression of anti-inflammatory cytokines and suppressed the expression of pro-inflammatory cytokines in Pseudomonas aeruginosa-infected macrophages and neutrophils.Flow cytometry data indicated that LiCl could promoted the apoptosis of Pseudomonas aeruginosa-infected macrophages and neutrophils.Conclusion LiCl inhibited the Pseudomonas aeruginosa-induced inflammation,via regulating the inflammatory cytokine expression and the apoptosis of inflammatory cells.
