Study on the correlation between MTHFR gene polymorphism and unexplained recurrent spontaneous abortion
10.3969/j.issn.1673-4130.2017.01.006
- VernacularTitle:MTHFR基因多态性与原因不明复发性流产的相关性研究
- Author:
Zhifen HUA
;
Caimei HUANG
;
Yanqiang LU
;
Ying LI
;
Bo GONG
;
Qi YANG
- Keywords:
unexplained recurrent spontaneous abortion;
methylenetetrahydrofolate reductase;
single nucleotide polymorphisms
- From:
International Journal of Laboratory Medicine
2017;38(1):16-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the correlation between MTHFR gene polymorphism and unexplained recurrent spontaneous abortion.Methods A case control study was used in this study,140 patients with unexplained recurrent spontaneous abortion(UR-SA) (abortion group)and 143 cases of normal women(control group)were recruited.Genomic DNA was obtained and extracted from the oral mucosa cells.Fluorescence quantitative PCR was used to examine the MTHFR gene polymorphisms,and Taqman-MGB technology was conducted to analysis the relationship between single nucleotide polymorphism and disease.Results There was statistically significant difference in the frequencies of C677T genotype and alleles between the two groups(P＜0.05).However,no significant difference in the frequencies of A1298C genotype and alleles between the two groups(P＞0.05).Conclusion MTHFR gene C677T polymorphism might be one of the genetic risk factors of URSA.
