Effect and mechanism of miRNA-146a on the proliferation and apoptosis of vascular smooth muscle cells

VernacularTitle:miRNA-146a调控血管平滑肌细胞增殖和凋亡的作用及机制
Author: Yunshan YANG
Keywords: RNA-146a; vascular smooth muscle cell; proliferation; apoptosis; mechanism
From: International Journal of Laboratory Medicine 2017;38(1):63-65
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of miRNA-146a on the proliferation and apoptosis of vascular smooth muscle cells and its mechanism.Methods Taking the SDP grade healthy male SD rats as the experimental subjects,After the digestion of vascular smooth muscle cells(VSMC),50 nmol/L transfected miRNA-146a antisense oligonucleotides,missense chain and the same dose of phosphate buffer(PBS)were compared.Results The number and absorbance value of VSMC in the experimental group were lower than those in the other two groups after transfection with 48 h,the apoptosis rate was significantly higher than that of the other two groups,the expression level of nuclear factor κBp65 and PCNA was lower than the other two groups,and the difference was statistically significant(P＜0.05).Conclusion miRNA-146a can promote the proliferation of VSMC,inhibiting the apoptosis of the cells,the mechanism of action is related to the increase of nuclear factor κBp65 and PCNA expression.