Neutralization of anti-venom IgY against main activities of Deinagkistrodon acutus venom
10.3969/j.issn.1001-1978.2017.01.011
- VernacularTitle:抗蛇毒IgY对尖吻蝮（Deinagkistrodon acutus）蛇毒主要活性的中和作用研究
- Author:
Jinhua LIU
;
Heng LI
;
Qiyi HE
;
Yingfeng ZHANG
;
Diancheng CHEN
;
Cong LUO
;
Xiaodong YU
- Keywords:
anti-D.acutus vemon IgY;
PLA2;
5′-nu-cleotidase;
hyaluronidase;
Serine proteinase (fibrino-genase);
metalloprotease;
ED50;
Neutralization
- From:
Chinese Pharmacological Bulletin
2017;33(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the potency of anti-D. acutus venom IgY neutralizing the main activities of D. acutus venom.Methods After mixing the different a-mounts of IgY with snake venom and incubating togeth-er,the main activities of snake venom were assayed by biochemical methods.Results The in vitro assays in-dicated that anti-D.acutus venom IgY obviously neu-tralized the activities of PLA2 ,5′-nucleotidase,hyalu-ronidase,metalloprotease and serine proteinase (fi-brinogenase)in D.acutus venom.Mouse experiments showed that the ED50 value of IgY for mouse was 1 131.09 μg.Conclusion Anti-D.acutus venom IgY antibodies have good effects in neutralizing D.acutus venom without the toxicities themselves.
