Establishment and evaluation of animal model of depression rats with kidney-yang deficiency
10.3969/j.issn.1001-1978.2017.01.024
- VernacularTitle:肾阳虚型抑郁症大鼠模型的建立和评价
- Author:
Shufen HE
;
Wenzheng JU
;
Haobin HU
;
Lijing ZHU
;
Guoliang DAI
;
Qian ZHANG
- Keywords:
depression;
kidney-yang deficiency;
hydrocorti-sone;
animal model;
index detection;
LC-MS/MS
- From:
Chinese Pharmacological Bulletin
2017;33(1):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish depression rat model with kidney-yang deficiency and evaluate the related parameters.Methods A total of 24 adult SD rats were divided into control group,mod-el group and self-healing group by body weight.Model group and self-healing group were injected hydrocortisone for 21 days, meanwhile the control group was injected sodium chloride.After modeling,the related indexs detection were taken out:open-field test,sucrose preference test and forced swim test.Then the orbital blood was taken to detect 5-HT,DA and NE by LC-MS/MS.And liver,kidney,spleen,thymus,adrenal gland,testis and epididymis were taken to calculate organ index.Results Compared with the control group,model rats displayed depres-sion and kidney yang deficiency syndrome such as dry hairs, chills,back arched and so on. Correspondingly, the body weight increased slowly.Immobility in FST was significantly in-creased. Sucrose preference, horizontal scores and vertical scores was significantly decreased.Organ index decreased in liv-er,kidney,spleen,adrenal,testis and epididymis.The level of 5-HT and NE was significantly high.Compared with the model group,5-HT,NE and DA were significantly reduced,5-HT and DA were even lower than those of the control group.Conclusion Depression rat model with kidney-yang deficiency could be in-duced by continuous injection of hydrocortisone.
