Study on in Vitro Screening and in Vivo Validation of Optimized Buyang Huanwu Decoction
10.3969/j.issn.1005-5304.2017.02.014
- VernacularTitle:补阳还五精简方的体外筛选及体内验证研究
- Author:
Xiuli ZHANG
;
Pan MENG
;
Yun XIANG
;
Chang LEI
;
Fang LIU
;
Dan HUANG
;
Chuan CAI
;
Guangxian CAI
;
Yuhong WANG
- Keywords:
Buyang Huanwu Decoction;
decomposed recipe;
MCAO model;
oxidative stress cell model;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(2):49-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the optimized Buyang Huanwu Decoction (BYHWD);To verify it. Methods H2O2 was used to induce PC12 cell oxidative stress models. MTT method was used to determine the prevention effects of BYHWD at different concentrations (0.1, 0.2, 0.5, 1.0, 2.0, 3.5 mg/mL) on in vitro oxidative stress cell models to define the optimized concentration. Orthogonal design was used to divide BYHWD single medicine into decomposed BYHWD groups, control group (only with DMEM), normal group (without H2O2 and medicine processing), and model group, to investigate the protective effects on PC12 cells. Optimized BYHWD was screened to decide the compatibility ratio of each medicine. MTT was used to detect the cell survival rate in each group. Middle cerebral artery occlusion was used to replicate MACO rat models. SD rats were randomly divided into sham-operation group, model group, BYHWD group and optimized BYHWD high-, medium-and low-dose groups. Each medication group was given relevant medicine for gavage. The screened results were verified. Results Compared with other decomposed BYHWD groups, the protective effects of the compatibility of Astragali Radix+Chuanxiong Rhizoma+Pheretima on PC12 cells was the best (P<0.05), which was nearly equaled to BYHWD. Compared with the model group, BYHWD and the optimized one could evidently reduce cerebral cortex infarction area and improve the impaired brain edema (P<0.05), and the medium-dose group was the best. Conclusion The optimized BYHWD ratio is:Astragali Radix:Chuanxiong Rhizoma:Pheretima=10:3:1.
