Application of ITS2 Barcoding in Identification of Oxytropis Falcate Bunge
10.3969/j.issn.1005-5304.2017.02.019
- VernacularTitle:应用ITS2条形码鉴定藏药镰形棘豆
- Author:
Conglin HUANG
;
Min GUO
;
Xiaolin WANG
;
Hua JIANG
- Keywords:
Oxytropis falcate Bunge;
DNA barcoding;
ITS2;
molecular identification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(2):76-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify Oxytropis falcate Bunge from its adulterants;To secure the quality and clinical efficacy of Oxytropis falcate Bunge. Methods Genomic DNA was extracted. ITS2 fragment of nuclear gene was amplified and sequenced. The homology of the sequences was analyzed, and the related data were analyzed by software MEGA. The NJ (neighbor-joining) tree was constructed based on ITS2 sequence. Results The intraspecific genetic distance of Oxytropis falcate Bunge (K2P distance) was 0, and the interspecific genetic distance (K2P distance) between Oxytropis falcate Bunge and its adulterants ranged among 0.068-0.084. The constructed NJ tree showed that ITS2 could identify Oxytropis falcate Bunge from its adulterants correctly. Conclusion ITS2 barcode can be used to identify Oxytropis falcate Bunge from its adulterants effectively, and this study provides important molecular evidence for the identification of Oxytropis falcate Bunge and safety in its clinical use.
