Effects of Heat-reinforcing Needling on Synovial Tissues of Rheumatoid Arthritis Rabbits ;with Cold Syndrome
10.3969/j.issn.1005-5304.2017.02.015
- VernacularTitle:热补针法对寒证类风湿关节炎模型家兔滑膜组织代谢物的影响
- Author:
Xiaozheng DU
;
Bo YUAN
;
Jinhai WANG
;
Xiaoli FANG
;
Xinghua ZHANG
;
Liang TIAN
;
Tingzhuo ZHANG
;
Xinglan LI
;
Huazong ZENG
- Keywords:
heat-reinforcing needling;
rheumatoid arthritis;
synovial tissue;
metabolomics;
GC-MS;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(2):55-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the endogenous metabolites in synovial tissues of rheumatoid arthritis (RA) rabbits with cold syndrome;To investigate the specificity mechanisms of heat-reinforcing needling for RA. Methods A total of forty healthy purple blue rabbits were randomly allocated to normal group, model group, reinforcing-reducing needling group (RRN), twirling-reinforcing needling group (TRN) and heat-reinforcing needling group (HRN) 8 rabbits in each group. Arthritis rabbits cold model was made with ovalbumin and freezing. Except for normal groupand model group, RRN was given acupuncture of reinforcing-reducing needling at Zusanli (ST36), TRN was given acupuncture of twirling-reinforcing needling at Zusanli (ST36), HRN was administrated acupuncture of heat-reinforcing needling at Zusanli (ST36), once a day and retaining 30 min, a total of seven days. Fresh synovial tissues of rabbits knee joints were extracted after the intervention, then GC-Q/TOF-MS technology were employed to evaluate metabolic profiles. Results The synovial tissues metabolites of TCA cycle, carbohydrate and fatty acid metabolism in model group mainly increased compared with normal group (P<0.05); The synovial tissues metabolites of various kinds above-mentioned decreased compared with model group in all intervention of acupuncture group (P<0.05), and the metabolites of TCA cycle and carbohydrate metabolism in HRN were obviously less than that of RRN and TRN (P<0.05). Conclusion The specificity of heat-reinforcing needling for RA manifests in regulation of TCA cycle and carbohydrate metabolism.
